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目的对豚鼠胰腺组织特络细胞(telocytes,TCs)的形态特征进行观察与分析,并探讨甲磺酸伊马替尼对TCs的干预作用。方法以健康9周龄豚鼠胰腺为研究对象,利用免疫组化染色对TCs免疫标志(c-kit/CD117、vimentin)进行观察分析。透射电镜观察TCs的超微结构。建立甲磺酸伊马替尼干预模型:豚鼠54只,雌雄不限,分正常对照组、用药组(7、14、21、28 d)、停药组(W7、W14、W21、W28 d);免疫组化染色观察用/停药后TCs变化;Western blot观察用/停药后胰腺组织内CD117、vimentin蛋白表达。结果免疫组化染色显示:胰腺内TCs位于结缔组织内,细胞呈现三角形及长梭形等多种形态,有一至多条长而细的细胞突起,突起包绕腺泡,并相连形成网络结构;电镜超微结构显示:TCs有念珠状的细长突起,核质比较高,胞质内含有少量的线粒体、滑面内质网等;甲磺酸伊马替尼干预后,TCs突起变短,网络结构减少,停药后逐渐恢复;用药后胰腺组织内c-kit/CD117和vimentin蛋白表达降低,停药后逐渐恢复,差异有统计学意义(P<0.05)。结论豚鼠胰腺内存在梭形有念珠状突起的TCs,TCs包绕腺泡形成网络结构;甲磺酸伊马替尼可影响胰腺内TCs的形态及蛋白表达。
Objective To observe and analyze the morphological characteristics of telocytes (TCs) in guinea pig pancreatic tissues and to explore the intervention effect of imatinib mesylate on TCs. Methods The pancreas of 9-week-old guinea pigs was used as the research object. Immunohistochemical staining was used to observe the immunological markers (c-kit / CD117, vimentin) of TCs. TEM observation of ultrastructure of TCs. Intervention model of imatinib mesylate was established: 54 guinea pigs were randomly divided into normal control group, medication group (7,14,21,28 d), withdrawal group (W7, W14, W21, W28 d) The changes of TCs were observed with / without stopping after immunohistochemical staining. The expressions of CD117 and vimentin in pancreatic tissues were observed by Western blot. Results Immunohistochemical staining showed that within the pancreas, the TCs were located in the connective tissue. The cells showed triangular and long fusiform morphology with one or more long and thin cell protrusions. The projections surrounded the acinus and connected to form a network structure. Electron microscopy The ultrastructure of TCs showed that there were rosette-like elongated protuberances with high nuclei, a small amount of mitochondria and synovial endoplasmic reticulum in the cytoplasm, TCs protuberances shortened after the intervention of imatinib mesylate, The structure was reduced and gradually recovered after stopping the treatment. The protein expression of c-kit / CD117 and vimentin in pancreas decreased after treatment, and gradually recovered after stopping the treatment. The difference was statistically significant (P <0.05). CONCLUSION TCs and foraminous mycelium are present in the pancreas of guinea pigs. TCs surround the acinus and form network structure. Imatinib mesylate can affect the morphology and protein expression of TCs in the pancreas.