国家重大科研仪器研制项目“超高时空分辨微型化双光子在体显微成像系统”(项目批准号:3132 7901)项目组于2013年成立。成立伊始,项目组全体成员便积极开展调研,探索如何将下一代的激光、扫描、控制、成像新原理和图像处理新方法等有机结合应用于在体荧光成像过程中。2013年11月,项目组发现了通过调制超声可调制梯度(TAG)透镜高速点扫描产生片层光的新原理。在国家重大科研仪器研制项目的支持下,项目组整合了光学仪器研发、电路控制、生物学实验等各方面的力量,调集关键设备、确定时间节点,群策群力,于2014年3月份完成了新型双光子光片显微镜,命名为2P3A- National major scientific research equipment development project “ultra-high spatio-temporal resolution miniaturized two-photon in vivo microscopy imaging system” (project approval number: 3132 7901) project team was established in 2013. At the beginning of its establishment, all members of the project team actively conducted research to explore how to apply the next generation of new principles of laser, scanning, control, imaging and new methods of image processing to in-vivo fluorescence imaging. In November 2013, the team found a new principle for producing sheet light by high-speed point-scan scanning of modulated ultrasound tunable gradient (TAG) lenses. With the support of major national scientific research equipment development projects, the project team integrated all aspects of optical instrument research and development, circuit control and biological experiments to mobilize key equipment, identify time points and make concerted efforts. In March 2014, the project completed a new type of double Photon light microscope, named 2P3A-