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本实验研究了大鼠烯丙基氯（ＣＰ）染毒神经纤维微管（ＭＴ）形态、单位面积内数量及其调节因子Ｃａ２＋、ＣａＭ、ｃＡＭＰ、ｃＧＭＰ含量；ＮＦ－ｍｙｏｓｉｎＡＴＰａｓｅ活性；以及ＣＰ和Ｃａ２＋对离体管蛋白组装的影响。结果发现，神经纤维ＭＴ变性、单位面积内ＭＴ数量减少或消失，具有时间效应关系；Ｃａ２＋增加、ＣａＭ减少、ｃＡＭＰ增加、ｃＧＭＰ减少、ｃＡＭＰ／ｃＧＭＰ比值增加，均有剂量效应关系；ＮＦ－ｍｙｏｓｉｎＡＴＰａｓｅ活性降低并有剂量效应关系；ＣＰ和Ｃａ２＋对离体管蛋白组装ＭＴ均有抑制作用并显现良好的剂量效应关系，但Ｃａ２＋在较低浓度（０．１２５ｍｍｏｌ／Ｌ）下即可抑制ＭＴ组装，而ＣＰ在较高浓度（０．１６３ｍｏｌ／Ｌ以上）时方能抑制ＭＴ组装。可以认为，ＣＰ抑制ＭＴ组装或促进ＭＴ去组装可能主要是通过使轴浆内Ｃａ２＋浓度增加所致。轴浆内Ｃａ２＋浓度增加与ＣａＭ结合成Ｃａ２＋－ＣａＭ复合物，一方面活化ＡＣ催化ＡＴＰ生成ｃＡＭＰ，再激活ＰＫＡ；另一方面活化ＣａＭ－ＰＫⅡ，活化的ＰＫＡ及ＣａＭ－ＰＫⅡ催化ＭＡＰ２磷酸化，抑制ＭＴ组装和促进ＭＴ去组装，造成ＭＴ变性和单位面积内数量减少或消失，以及ＮＦ－ｍｙｏｓｉｎＡＴＰａｓｅ活?
In this study, we investigated the morphological changes of the microtubules (MTs) in the rat alveolar chloride (CP) group, the content of Ca2 +, CaM, cAMP and cGMP, the activity of NF-myosin ATPase and the activities of CP and Ca2 + Effect on the tube assembly of the tube. The results showed that there was a time-dependent relationship between the amount of MT and the number of MT in per unit area. The Ca2 + increased, CaM decreased, cAMP increased, cGMP decreased, cAMP / cGMP ratio increased, and dose-effect relationship was observed. NF-myosin ATPase activity (P <0.05). The inhibitory effect of CP and Ca2 + on the assembly of MT in vitro and the good dose-response relationship were observed. However, Ca2 + inhibited MT assembly at a lower concentration (0.125 mmol / L) CP at a higher concentration (0.163mol / L above) can inhibit MT assembly. It is believed that the inhibition of MT assembly or the promotion of MT disassembly by CP may be mainly caused by the increase of intracellular concentration of Ca2 +. On the other hand, activation of CaM-PKⅡ, activation of PKA and CaM-PKⅡ catalyze the phosphorylation of MAP2, and inhibit the phosphorylation of MAP2 by Ca2 + -Ma complex. MT assembly and to promote MT to assemble, resulting in MT degeneration and the number of units within the area to reduce or disappear, and NF-myosinATPase activity?