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本实验研究了大鼠烯丙基氯(CP)染毒神经纤维微管(MT)形态、单位面积内数量及其调节因子Ca2+、CaM、cAMP、cGMP含量;NF-myosinATPase活性;以及CP和Ca2+对离体管蛋白组装的影响。结果发现,神经纤维MT变性、单位面积内MT数量减少或消失,具有时间效应关系;Ca2+增加、CaM减少、cAMP增加、cGMP减少、cAMP/cGMP比值增加,均有剂量效应关系;NF-myosinATPase活性降低并有剂量效应关系;CP和Ca2+对离体管蛋白组装MT均有抑制作用并显现良好的剂量效应关系,但Ca2+在较低浓度(0.125mmol/L)下即可抑制MT组装,而CP在较高浓度(0.163mol/L以上)时方能抑制MT组装。可以认为,CP抑制MT组装或促进MT去组装可能主要是通过使轴浆内Ca2+浓度增加所致。轴浆内Ca2+浓度增加与CaM结合成Ca2+-CaM复合物,一方面活化AC催化ATP生成cAMP,再激活PKA;另一方面活化CaM-PKⅡ,活化的PKA及CaM-PKⅡ催化MAP2磷酸化,抑制MT组装和促进MT去组装,造成MT变性和单位面积内数量减少或消失,以及NF-myosinATPase活?
In this study, we investigated the morphological changes of the microtubules (MTs) in the rat alveolar chloride (CP) group, the content of Ca2 +, CaM, cAMP and cGMP, the activity of NF-myosin ATPase and the activities of CP and Ca2 + Effect on the tube assembly of the tube. The results showed that there was a time-dependent relationship between the amount of MT and the number of MT in per unit area. The Ca2 + increased, CaM decreased, cAMP increased, cGMP decreased, cAMP / cGMP ratio increased, and dose-effect relationship was observed. NF-myosin ATPase activity (P <0.05). The inhibitory effect of CP and Ca2 + on the assembly of MT in vitro and the good dose-response relationship were observed. However, Ca2 + inhibited MT assembly at a lower concentration (0.125 mmol / L) CP at a higher concentration (0.163mol / L above) can inhibit MT assembly. It is believed that the inhibition of MT assembly or the promotion of MT disassembly by CP may be mainly caused by the increase of intracellular concentration of Ca2 +. On the other hand, activation of CaM-PKⅡ, activation of PKA and CaM-PKⅡ catalyze the phosphorylation of MAP2, and inhibit the phosphorylation of MAP2 by Ca2 + -Ma complex. MT assembly and to promote MT to assemble, resulting in MT degeneration and the number of units within the area to reduce or disappear, and NF-myosinATPase activity?