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DAB(Diaminobenzidine,二氨基联苯胺)为免疫组织化学技术中最常用的显色物质,粉末状,有致癌性,每次用量很少(毫克计),以往须在使用前临时配制成新鲜液体,因此有诸多不便。我们设计实验了DAB液配制保存与使用的新方法,效果理想,报告如下。实验方法一、DAB液的配制与保存用普通天平一次秤取DAB100mg,加PBS液20.5ml,于冷环境(4—10℃)下用磁力搅拌器使其溶解,10分钟。普通离心机离心2分钟,将上清液分装入20支密封口的塑料管(每支1ml,含DAB5mg)。在普通
DAB (Diaminobenzidine, diaminobenzidine) is the most commonly used colorimetric substance in immunohistochemical technology. It is powdery and carcinogenic. It is used in small amounts (in milligrams). In the past, it was necessary to prepare fresh liquids before use. Therefore, there are many inconveniences. We designed and tested a new method for the preparation, storage, and use of DAB solutions. The results are satisfactory. The report is as follows. Experimental Method I. Preparation and storage of DAB solution DAB 100 mg was weighed once with an ordinary balance, and 20.5 ml of PBS solution was added. The solution was dissolved in a cold environment (4-10° C.) with a magnetic stirrer for 10 minutes. Ordinary centrifuge centrifugation for 2 minutes, the supernatant was distributed into 20 sealed plastic tubes (each 1ml, containing DAB 5mg). In the ordinary