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目的体外培养的人腹膜间皮细胞（ＨＰＭＣ）是研究腹膜功能的有用模型，我们为此建立了一种改良消化法，以从人大网膜分离腹膜间皮细胞。方法用０．２％胰蛋白酶－０．０２％ＥＤＴＡＮａ２消化人大网膜组织碎片，细胞悬液经１００目筛网滤过后进行培养：瑞士姬姆萨染色观察细胞形态，计算细胞纯度：免疫组化（ＡＢＣ法）鉴定细胞。结果分离培养的细胞光镜下呈铺路鹅卵石状，纯度达９５％以上。细胞表面标志鉴定显示角蛋白、波形蛋白抗原阳性，第Ⅷ因子相关抗原、白细胞ＣＤ４５抗原阴性，证实分离培养的确是ＨＰＭＣ。结论胰蛋白酶消化法是一种简单实用的分离ＨＰＭＣ的方法。
Purpose Human in vitro cultured human peritoneal mesothelial cells (HPMC) is a useful model for studying peritoneal function, and we have established an improved digestion method to separate peritoneal mesothelial cells from the human omental. Methods Human omental tissue fragments were digested with 0.2% trypsin-0.02% EDTANa2 and the cell suspension was filtered through a 100-mesh sieve and cultured. The morphology of the cells was observed by Giemsa staining and the cell purity was calculated: immunohistochemistry (ABC method) to identify cells. Results The cultured cells were paved with pebbles under the light microscope with the purity of over 95%. Identification of cell surface markers showed that keratin, vimentin antigen-positive, factor Ⅷ related antigen, leukocyte CD45 antigen negative, proved isolated culture is indeed HPMC. Conclusion Trypsin digestion is a simple and practical method for the isolation of HPMC.