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目的优化外周血Ⅰ型干扰素细胞(IPC)检测方法,分析我国正常人及 HIV 感染者外周血 IPC 水平。方法以 BDCA_2、BDCA_4及 CD_4为新的 IPC 特征性表面标志组合优化 IPC 全血检测方法,应用该方法测定49份正常人和36份 HIV 感染者外周血 IPC 水平。结果 IPC 绝对计数全血染色组显著高于 PBMC 染色组(t=-4.911,P<0.01),两者分别为(1.15±0.87)个/μl和(3.00±1.70)/μl;淋巴细胞和单核细胞设门与全白细胞设门方法所得结果高度相关(r~2=0.956,P<0.01);样品在室温和4℃环境下25 h 内检测结果无显著性变化。HIV 感染者和正常人外周血 IPC 水平平均值分别为6.44个/μl和2.96个/μl,CD_4细胞水平平均值分别为874.92个/μl和551.5个/μl ,差异均有统计学意义(P<0.01)。结论该方法特异性高,重复性好,操作简便。初步结果显示我国 HIV 感染者外周血 IPC 水平显著低于正常人群。
Objective To optimize the detection of peripheral blood type I interferons (IPC) and analyze the levels of peripheral blood IPC in normal and HIV-infected people in China. Methods IPC whole blood detection method was optimized by combining BDCA_2, BDCA_4 and CD_4 with new IPC surface markers. The IPC method was used to determine the level of IPC in 49 normal subjects and 36 HIV infected individuals. Results Compared with PBMC staining group (t = -4.911, P <0.01), the absolute counts of whole blood in IPC group were (1.15 ± 0.87) / μl and (3.00 ± 1.70) / μl, respectively; lymphocytes and single The results of nucleated cell gate and all-leukocyte gating method were highly correlated (r ~ 2 = 0.956, P <0.01). There was no significant change in the test results within 25 h at room temperature and 4 ℃. The mean levels of IPC in peripheral blood of HIV-infected and normal individuals were 6.44 / μl and 2.96 / μl, respectively. The average CD_4 cell levels were 874.92 / μl and 551.5 / μl, respectively, with statistical significance (P < 0.01). Conclusion The method is highly specific, reproducible and easy to operate. The preliminary results show that the level of IPC in peripheral blood of HIV-infected people in our country is significantly lower than that of normal people.