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本文作者主要报道了一种测定ＤＮＡ聚合酶β活力的方法，其基本原理是：ＤＮＡ聚合酶α、β等能催化活化ＤＮＡ中的ＤＮＡ合成，将３Ｈ－ＴＴＰ等掺入ＤＮＡ双链缺口中。在选用对ＤＮＡ聚合酶β活力无影响，而对其它ＤＮＡ聚合酶活力有选择性抑制作用的抑制剂Ｎ－乙基马来酰亚胺以后，活化ＤＮＡ中３Ｈ－ＴＴＰ掺入量可以代表ＤＮＡ聚合酶β活力的大小，用该法测定裸鼠肝细胞、肝癌细胞核内ＤＮＡ聚合酶β活力，发现肝癌细胞内ＤＮＡ聚合酶β活力高于正常肝细胞。经γ射线照射３０Ｇｙ后，两种细胞核内该酶活力均无明显下降，本方法操作简单、比较经济、准确可靠，易于在国内推广应用。
The author of this paper mainly reported a method for determining the activity of DNA polymerase β. The basic principle is that DNA polymerase α, β, etc. catalyze the DNA synthesis in activated DNA, and incorporate 3H-TTP into DNA double-stranded gaps. The 3H-TTP incorporation in the activated DNA after the selection of N-ethylmaleimide, an inhibitor that has no effect on the activity of DNA polymerase β but selectively inhibits the activity of other DNA polymerases, Enzyme β activity of the size of the method used to determine the nude mouse liver cells, liver cancer cells DNA polymerase β activity and found that liver cancer cells DNA polymerase β activity than normal liver cells. The γ-ray irradiation 30Gy, the two nuclei within the enzyme activity was no significant decline, the method is simple, more economical, accurate and reliable, easy to promote the use of the country.