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水稻是最重要的粮食作物之一,在中国水稻产量占粮食总产的44%,武育粳7号是人们广泛种植的高产水稻品种之一。本实验室前期研究表明Os NRT2.3b是水稻中一个硝酸盐运输蛋白。本研究通过构建Os NRT2.3b在武育粳7号中的超表达材料,通过一系列的分子和生理实验,发现Os NRT2.3b超表达材料在武育粳7号中,有效分蘖数明显增多,促进株高和有效穗的生长发育。转基因材料的产量在不同氮素水平下均要高于野生型,并且在中氮(135 kg·N·hm-2)条件下增产效果最明显,平均增产比例约为22.78%。经过对T2、T3两代田间小区试验的产量分析,发现369株系在中氮(135 kg·N·hm-2)条件下增产效果最明显,两年平均比野生型武育粳7增产了38.8%。研究结果说明过量表达的Os NRT2.3b在低中氮肥条件下可以使武育粳7号的产量增加20%以上。
Rice is one of the most important grain crops, accounting for 44% of the total grain output in China. Wuyujing 7 is one of the high-yielding rice varieties widely planted. Our previous study showed that Os NRT2.3b is a nitrate transport protein in rice. In this study, we constructed Os NRT2.3b overexpression material in Wuyujing 7 through a series of molecular and physiological experiments and found that Os NRT2.3b overexpression material in Wuyujing 7 significantly increased the number of effective tillers , Promote plant height and effective spike growth and development. The yield of transgenic material was higher than that of wild type at different nitrogen levels, and the yield was most obvious under medium nitrogen (135 kg · N · hm-2). The average yield was about 22.78%. After analyzing the yield of T2 and T3 field plots, it was found that 369 lines had the most obvious yield increase under medium nitrogen (135 kg · N · hm-2), and the average yield of 369 lines was higher than that of wild type Wuyujing 7 38.8%. The results showed that the overexpression of Os NRT2.3b could increase the yield of Wuyujing 7 by more than 20% under low and medium nitrogen conditions.