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目的观察马钱子碱中毒后不同时间、不同剂量Bcl-2和Caspase-3表达的变化。方法选用20只Wistar大鼠为正常对照组,44只Wistar大鼠复制马钱子中毒模型,应用免疫组织化学和图像分析方法研究大鼠脑神经细胞马钱子中毒后Bcl-2与Caspase-3蛋白表达的变化规律。结果大鼠在马钱子中毒低、中剂量后的1~7 d不同时间段内,脑神经细胞Bcl-2与Caspase-3蛋白阳性表达细胞数均高于正常对照组,与相应的对照组比较,差异均有统计学意义(P<0.05)。结论Bcl-2与Caspase-3参与了马钱子中毒的病理生理过程。
Objective To observe the changes of Bcl-2 and Caspase-3 expression at different times after strychnine poisoning. Methods 20 Wistar rats were selected as the normal control group and 44 Wistar rats were used to establish the model of Strychnos poisoning. The expressions of Bcl-2 and Caspase-3 were detected by immunohistochemistry and image analysis Protein expression changes. Results The number of Bcl-2 and Caspase-3 positive cells in brain neurons was significantly higher than that in the normal control group at different time points from 1 to 7 days after hypoxia poisoning. Compared with the corresponding control group Comparison, the differences were statistically significant (P <0.05). Conclusion Bcl-2 and Caspase-3 are involved in the pathophysiological process of Strychnos poisoning.