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目的 探讨内皮素-1(ET-1)在烫(烧)伤大鼠脑血管损伤的表达及其作用.方法 应用组织学方法观察8只烫伤大鼠脑基底动脉组织结构的变化;应用RT-PCR测定48只烫伤大鼠脑基底动脉ET-1 mRNA的表达;应用Western blotting分析48只烫伤大鼠脑基底动脉ET-1的表达;应用放射免疫分析检测48只烫伤大鼠脑基底动脉ET-1 水平的变化.结果 烫伤大鼠脑基底动脉呈现病理学组织结构变化;烫伤后3h大鼠脑基底动脉ET-1 mRNA表达较正常对照组开始升高,6h 达高峰;烫伤后6h大鼠脑基底动脉ET-1 表达较正常对照组增强,12h 达高峰;烫伤后各组大鼠脑基底动脉ET-1水平较正常对照组明显升高,6h 达高峰.结论 烫(烧)伤可诱发大鼠脑血管ET-1表达增加,增加表达的ET-1可能与烫(烧)伤大鼠脑血管损伤的病理发生有关.“,”Objective To investigate the expression and effects of endothelin-1 (ET-1) in cerebral vessels of scalded rats. MethodHistological method was used to observe the alteration of histological structure of basilar artery in 8 scalded rats. Reverse transcription polymerase chain reaction(RT-PCR) was performed to examine expression of ET-1 mRNA;Western blotting to analyse expression of endothelin-1;radioimmunoassay to measure ET-1 levels;R3espectively, of cerebral basilar artery in 48 scalded rats. ResultVascular pathohistological changes were observed in cerebral basilar artery after rats were scalded;ET-1, ET-1 mRNA expression and ET-1 levels of basilar artery in scald 6 hours, 12 hours and 24 hours group were obviously higher than that of control group. Conclusion Scald may cause an increases of ET-1 expression in basilar artery, and elevated ET-1 expression may relate to the pathogenesis of cerebral vascular injury in scalded rats.