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目的:探讨贯叶连翘提取物对百草枯诱导的大鼠肺纤维化模型的干预作用及机制。方法:20只Sprague-dawly大鼠随机等分为4组:百草枯组,贯叶连翘提取物组,百草枯+贯叶连翘提取物组和对照组。百草枯液按80 mg/kg一次性灌胃,贯叶连翘提取物按400 mg/kg灌胃,连用3 d,对照组仅用生理盐水。于21 d处死后取肺脏行HE染色鉴定,并用碱水解法测羟脯氨酸含量,同时以八木国夫荧光法测定肺组织脂质过氧化产物,盐酸羟胺法测定组织总超氧化物歧化酶的酶活力。结果:百草枯染毒动物可致肺纤维化,羟脯氨酸和丙二醛含量明显增高。百草枯+贯叶连翘提取物组与百草枯组比较,第21天时肺纤维化减轻,羟脯氨酸和丙二醛含量减少(P<0.05),与对照组相比总超氧化物歧化酶无明显变化(P>0.05)。结论:贯叶连翘提取物对百草枯诱导的大鼠肺纤维化模型有抑制作用,其机制可能与抑制脂质过氧化有关。
OBJECTIVE: To investigate the effect and mechanism of Hypericum perforatum extract on paraquat-induced pulmonary fibrosis in rats. Methods: Twenty Sprague-dawly rats were randomly divided into four groups: paraquat group, Hypericum perforatum, Paraquat + Hypericum perforatum, and control group. Paraquat was given at a single dose of 80 mg/kg, and St. John’s wort extract was given at 400 mg/kg for 3 days. The control group used only normal saline. After 21 days of death, the lungs were stained with HE for identification, and the content of hydroxyproline was measured by alkali hydrolysis. At the same time, lipid peroxidation products in the lung tissue were measured by Yagi Koshihikari method. The total superoxide dismutase was determined by hydroxylamine hydrochloride method. Enzyme activity. RESULTS: Paraquat-infected animals could cause pulmonary fibrosis and the levels of hydroxyproline and malondialdehyde increased significantly. Paraquat + St John’s wort extract group compared with paraquat group, lung fibrosis was reduced on the 21st day, hydroxyproline and malondialdehyde content was reduced (P <0.05), compared with the control group, total superoxide dismutase, no Significant changes (P>0.05). Conclusion: Hypericum perforatum extract has inhibitory effects on paraquat-induced pulmonary fibrosis in rats, and its mechanism may be related to the inhibition of lipid peroxidation.