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目的:探讨烟雾吸入导致大鼠急性肺损伤(ALI)时血清TNF-α、IL-8含量变化及卡托普利对ALI和TNF-α、IL-8的影响。方法:36只健康成年Wistar大鼠随机平均分成正常对照组、烟雾致伤组(ALI组)和卡托普利干预组(CAP组)。CAP组腹腔注射卡托普利(5mg/kg),对照组和ALI组则腹腔注射等量生理盐水。15min后复制大鼠重度烟雾吸入性肺损伤模型。致伤后5 min、5h分别采动脉血进行血气分析.检测烟雾致伤后5h血清TNF-α、IL-8含量,光镜观察肺病理形态学变化。结果:ALI组致伤后5h血清TNF-α、IL-8含量明显高于正常对照组( P<0.01),肺组织病理学改变明显;CAP组致伤后5h血清IL-8含量显著低于ALI组(P<0.05),肺损伤相对较轻。结论:TNF-α、IL-8可能在烟雾吸入性ALI中起重要作用,卡托普利能降低大鼠ALI时血清IL-8浓度,减轻烟雾吸入致ALI时的肺组织损伤。
Objective: To investigate the changes of serum levels of TNF-α and IL-8 in smoke-induced acute lung injury (ALI) and the effects of captopril on ALI, TNF-α and IL-8. Methods: Thirty - six healthy adult Wistar rats were randomly divided into normal control group, smoke injury group (ALI group) and captopril intervention group (CAP group). Captopril (5 mg / kg) was administered intraperitoneally to CAP group, while saline and saline were injected into control group and ALI group. Rat model of severe smoke inhalation lung injury was reproduced after 15 minutes. At 5 and 5 hours after injury, arterial blood was collected for blood gas analysis, serum levels of TNF-α and IL-8 were detected 5 h after smoke-induced injury, and pathological changes of lung were observed by light microscope. Results: The levels of serum TNF-α and IL-8 in ALI group were significantly higher than those in normal control group (P <0.01), and the pathological changes were obvious in ALI group. The serum IL-8 level in CAP group was significantly lower than that of normal group ALI group (P <0.05), lung injury is relatively light. CONCLUSION: TNF-α and IL-8 may play an important role in smoke-induced ALI. Captopril can decrease the concentration of serum IL-8 in ALI and attenuate lung injury induced by smoke inhalation.