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The No.601 watermelon (citrullus lanatus) seeds were treated with 25 keV N+ implantation at the dosage of 7.8*10 ions/cm2 . After treatment, watermelon seeds were incubated with 380 *g/*l pumpkin (Cucubita, maxima Duch) DNA solution at 35* for 5 hours. By two-generations of selection and resistance screening at seedling stage, one transformed material was selected out, whose rind color is similar to that of the donor pumpkin and whose size of seeds is between that of the donor and the receptor. Using AFLP (amplified fragment length polymorphism) technique, two polymorphic DNA fragments were amplified. This primarily testified that the donor DNA fragments/gene were introduced into the receptor cell and integrated into the genomic DNA of the receptor.
After treatment, watermelon seeds were incubated with 380 * g / * l pumpkin (Cucubita, maxima Duch) DNA. The No. 601 watermelon (citrullus lanatus) seeds were treated with 25 keV N + implantation at the dosage of 7.8 * 10 ions / By two-generations of selection and resistance screening at seedling stage, one transformed material was selected out, whose rind color is similar to that of the donor pumpkin and whose size of seeds is between that of the donor And the receptor. Using AFLP (amplified fragment length polymorphism) technique, two polymorphic DNA fragments were amplified. This injected testified that the donor DNA fragments / gene were introduced into the receptor cell and integrated into the genomic DNA of the receptor.