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目的测定多发性硬化(MS)患者记忆性 T 细胞亚群的水平,并探讨可能导致记忆性 T细胞亚群变化的机制。方法采用流式细胞仪和酶联免疫吸附分析(ELISA)分别检测未治疗 MS、脑梗死和正常对照组的外周血记忆性 T 细胞亚群表型和血浆白细胞介素-15(IL-15)浓度。结果 MS患者 CD8+中心记忆性 T 细胞(TCM)明显高于正常对照组(20%±11%和13%±6%,P<0.05),CD8+终末效应记忆性 T 细胞显著少于正常对照组(24%±15%和39%±19%,P<0.05);MS 患者血浆 IL-15水平较正常对照显著升高(36.01pg/ml和9.53 pg/ml,P<0.05)。结论 MS 患者外周血CD8+TCM 的上调可能反映了在 MS 早期被诱导产生的一个持续的慢性炎性应答,而 IL-15则可能参与了促进 TCM 分化的调节过程。
Objective To determine the level of memory T cell subsets in patients with multiple sclerosis (MS) and to explore mechanisms that may contribute to the alteration of memory T cell subsets. Methods The phenotypes of peripheral blood T cell subsets and plasma interleukin-15 (IL-15) in untreated MS, cerebral infarction and normal control group were detected by flow cytometry and enzyme-linked immunosorbent assay (ELISA) concentration. Results The CD8 + central memory T cells (TCM) of MS patients were significantly higher than those of the normal controls (20% ± 11% and 13% ± 6%, P <0.05), while the number of CD8 + T memory memory terminals was significantly lower than that of the normal controls (24% ± 15% vs 39% ± 19%, P <0.05). The levels of plasma IL-15 in MS patients were significantly higher than those in normal controls (36.01pg / ml and 9.53 pg / ml, P <0.05). Conclusion The upregulation of CD8 + TCM in peripheral blood of patients with MS may reflect a persistent chronic inflammatory response induced in early stages of MS. IL-15 may be involved in the regulation of TCM differentiation.