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对四株蓝氏贾第虫[三株分离自中国(C1、C2、C3)],一株分离自美国(CDC)]的可溶性蛋白和同工酶分别用SDS—PAGE和PAGE法进行分析。结果表明,每一受试虫株在SDS—PAGE中均显示出众多蛋白带,其主带数目分别为9、13、13、11。大多数带的分子量介于17.5KDa~94KDa之间。同工酶分析结果表明,在酯酶(EST)分析中,CDC出现4条带,其中3条深染。C3呈现3条浅带,但C1和C2未见条带出现。在酸性磷酸酶(AP)分析中,三个中国虫株均各出现1条浅带和1条深带,而在CDC中只出现1条。在苹果酸脱氢酶(MDH)分析中,C1可见1条带,而在C3为2条,C2和CDC未见条带。在苹果酸酶(ME)分析中,中国三个虫株均各出现1条带,而CDC则无
Four isolates of Giardia lamblia [three isolates from China (C1, C2, C3)] and one isolator from the USA (CDC) were analyzed by SDS-PAGE and PAGE, respectively. The results showed that each strain tested showed many protein bands in SDS-PAGE, the main bands were 9,13,13,11 respectively. Most bands have molecular weights between 17.5 and 94 kDa. Isozyme analysis showed that there were 4 bands in CDC and 3 of them were deep-stained in EST analysis. C3 showed three shallow bands, but no bands C1 and C2 appeared. In the analysis of acid phosphatase (AP), there were 1 shallow zone and 1 deep zone in each of three Chinese insect strains, and only 1 in CDC. In malate dehydrogenase (MDH) analysis, one band was observed for C1, two bands for C3 and no bands for C2 and CDC. In the malic acid enzyme (ME) analysis, there were 1 band in each of the three Chinese strains, while none in CDC