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目的:探讨胚胎培养液中低密度脂蛋白受体相关蛋白-1(LRP-1)的表达与胚胎形态学评分及其与移植后妊娠率的关系。方法:收集培养第3天可利用胚胎的培养液,用ELISA方法检测其中LRP-1的表达水平,比较不同胚胎形态学评分间胚胎培养液中LRP-1水平的差异,并比较妊娠组与未妊娠组间移植胚胎培养液中LRP-1水平。结果:(1)优质胚胎对应的培养液中LRP-1的表达水平高于Ⅲ级胚胎的表达水平[(991.79±182.60)pg/ml vs(639.22±108.09)pg/ml],差异具有统计学意义(P<0.05);(2)妊娠组胚胎培养液中LRP-1的表达水平[(1 056.34±166.82)pg/ml]高于未妊娠组的表达水平[(899.46±169.37)pg/ml],差异具有统计学意义(P<0.05)。结论:胚胎培养液中LRP-1的表达水平可作为预测胚胎发育潜能及妊娠结局的参考指标之一。
Objective: To investigate the relationship between low density lipoprotein receptor-associated protein-1 (LRP-1) expression and embryo morphology score and its relationship with post-transplant pregnancy rate in embryo culture medium. Methods: Cultured embryos were collected on day 3, and the expression of LRP-1 was detected by ELISA. The differences of LRP-1 levels in embryo culture medium between different embryo morphological scores were compared. LRP-1 levels in embryo culture fluid during pregnancy. Results: (1) The expression level of LRP-1 in high quality embryos was higher than that in grade Ⅲ embryos [(991.79 ± 182.60) pg / ml vs (639.22 ± 108.09) pg / ml] (P <0.05). (2) The expression level of LRP-1 in embryo culture group was higher than that in non-pregnancy group [(1 056.34 ± 166.82) pg / ml] [(899.46 ± 169.37) pg / ml ], The difference was statistically significant (P <0.05). Conclusion: The expression level of LRP-1 in embryo culture fluid can be used as a reference index for predicting embryonic development potential and pregnancy outcome.