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本文利用酶连免疫吸着测定法(ELISA)来测定普通小麦Fukuho(简自Fukuhomugi)、阿尔泰黑麦Pilger及其杂交F_1(3个材料均于2-3叶期接种)叶片中大麦黄矮病毒(BYDV)含量。结果表明窄颖赖草(Leymus angustus)对BYDV具有免疫性和高抗性。在Fukuho中,该病毒显著增殖;在大田栽培条件下,Fukuho对大麦黄矮病表现为中等水平的耐性。单倍体杂交种F_1代“Fukuho×窄颖赖草”几乎同其野生亲本表现为相同的抗性,F_1在接种后的16 d内BYDV含量很低,再后就不含该病毒。窄颖赖草对BYDV具抗性及F_1杂交种也同样具有这种抗性特征,这一发现为扩大小麦的BYDV抗性基因库提供了抗源材料。
In this study, enzyme-linked immunosorbent assay (ELISA) was used to determine the relative abundance of barley yellow dwarf virus (barley yellow dwarf virus) in leaves of common wheat Fukuho (Fukuhomugi), Altai rye Pilger and its hybrid F_1 (all three were inoculated 2-3 days) BYDV) content. The results show that Leymus angustus is immunogenic and highly resistant to BYDV. The virus significantly proliferated in Fukuho; Fukuho showed moderate level of tolerance to barley yellow dwarf virus under field conditions. The haploid hybrid F 1, “Fukuho × Narretsii,” showed almost the same resistance as its wild counterparts. The BYDV content of F_1 was very low within 16 days after inoculation, and was not included in the virus. The resistance of BYDV-resistant and F1 hybrids was also found to be resistant to this resistance. This finding provides a source material for expanding the BYDV resistance gene pool in wheat.