论文部分内容阅读
目的通过毛细管区带电泳(HPCE)内标法,比较戊己丸传统汤剂、配方颗粒汤剂、复方颗粒汤剂中盐酸小檗碱、盐酸巴马汀、盐酸药根碱、芍药苷成分量差异。方法采用HPCE法,以50 mmol/L硼砂缓冲液-甲醇(2∶1)的溶液作为缓冲液,工作电压为25 kV,压力进样5 kPa×15 s,电解质封口:5 kPa×10 s;柱温15℃。结果该方法简便、准确,其中盐酸小檗碱、盐酸巴马汀、盐酸药根碱3种生物碱的量在配方颗粒汤剂中明显高于传统汤剂与复方颗粒汤剂,芍药苷则是在传统汤剂中量最高。结论本法对戊己丸配方和复方颗粒剂在临床的推广以及质量控制具有指导价值。
OBJECTIVE To compare the contents of traditional Chinese herbal medicine, such as traditional Chinese decoction of Wuji Pills, berberine hydrochloride, berberine hydrochloride, palmatine hydrochloride, jatrorrhizine hydrochloride and paeoniflorin by capillary zone electrophoresis (HPCE) difference. Methods A 50 mmol / L borax buffer - methanol (2: 1) solution was used as the buffer. The working voltage was 25 kV, the pressure was injected for 5 kPa × 15 s, and the electrolyte was sealed for 5 kPa × 10 s. Column temperature 15 ℃. Results The method was simple and accurate. The contents of three alkaloids, berberine hydrochloride, palmatine hydrochloride and jatrorrhizine hydrochloride were significantly higher than that of the traditional decoction and the compound granule decoction in the formula granule decoction. Paeoniflorin was In traditional decoction in the highest volume. Conclusions This method is of guiding value to the promotion and quality control of Wuji Pills and compound granules.