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目的：探索一种便捷的血吸虫病诊断方法。方法：在原已建立的ＥＬＩＳＡ法的基础上，应用３个针对日本血吸虫抗原的不同表位的单克隆抗体标记胶体金，建立检测日本血吸虫病患者血清中血吸虫抗原的斑点金免疫渗滤法。抗原与抗体通过渗滤在硝酸纤维薄膜上进行反应，数分钟用肉眼观察结果。结果：本法检测ＳＥＡ的敏感度为１６ｎｇ／ｍｌ，在６９例慢性日本血吸虫病患者血清的检测中，阳性率为６０．８％，特异性为９５．２％；同时用ｄｏｔ－ＥＬＩＳＡ检测１３７例慢性日本血吸虫病患者，阳性率为５４．７％、特异性为９４．６％；夹心ＥＬＩＳＡ检测１１８例慢性日本血吸虫病患者，阳性率为６１．９％、特异性为９５．７％。结论：经数理统计Ｇ检验证实斑点金免疫渗滤法的敏感性和特异性同ＥＬＩＳＡ相近，且操作更简便且快速。
Objective: To explore a convenient method of diagnosis of schistosomiasis. Methods: Based on the established ELISA method, three monoclonal antibodies against different epitopes of Schistosoma japonicum antigen were used to label colloidal gold to establish a dot-immunogold filtration assay for the detection of schistosoma antigen in sera of patients with schistosomiasis japonica. Antigen and antibody were diafiltered on nitrocellulose membrane for reaction for several minutes with naked eyes. Results: The sensitivity of this method for the detection of SEA was 16 ng / ml. In the sera of 69 patients with chronic schistosomiasis japonica, the positive rate was 60.8% and the specificity was 95.2%. Meanwhile, Dot-ELISA 137 In patients with chronic schistosomiasis japonica, the positive rate was 54.7% and the specificity was 94.6%. The sandwich ELISA was used to detect 118 cases of chronic schistosomiasis japonica, with a positive rate of 61.9% and a specificity of 95.7%. CONCLUSIONS: The G-test confirms the sensitivity and specificity of Auger Auger’s immunospot assay to ELISA and is easier and faster to operate.