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目的:从TH1/TH2细胞平衡角度观察清肺口服液对感染RSV肺炎小鼠肺组织内T-bet、GATA3及IL-12的影响,并对其作用机制进行初步探讨。方法:将45只BABL/c小鼠随机分为5组,正常对照组,模型组,利巴韦林组,清肺(3.4 g/mL)组,清肺(10.2 g/mL)组。每组9只,用RSV(Long株)病毒予小鼠滴鼻造模,病毒激发24 h后,分给药72 h后处死小鼠,立即取小鼠肺组织行病理分析,及肺细胞中RT-PCR检测T-bet和GATA3蛋白表达水平,ELISA方法检测肺泡灌洗液(BALF)中IL-12的含量,流式细胞术方法检测小鼠脾脏细胞IL-4和INF-γ表达含量。结果:小鼠模型组与各给药组小鼠肺组织均有T-bet和IL-12(P<0.05,P<0.01)显著升高,GATA3显著降低。结论:清肺口服液具有调节TH1/TH2平衡,并对于感染rsv小鼠体内T-bet及IL-12细胞因子具有明显提升作用,而对GATA3则有明显抑制效果,从而进一步证明了清肺口服液治疗RSV病毒性肺炎有效性。
OBJECTIVE: To observe the effects of Qingfei Oral Liquid on the expression of T-bet, GATA3 and IL-12 in the lung tissue of mice infected with RSV pneumonia from the perspective of the balance of TH1 / TH2 cells and to explore its mechanism. Methods: Forty five BABL / c mice were randomly divided into 5 groups: normal control group, model group, ribavirin group, Qingfei group (3.4 g / mL) and Qingfei group (10.2 g / mL) The mice in each group were injected intranasally with RSV (Long strain) virus. After being challenged with the virus for 24 hours, the mice were sacrificed and the mice were sacrificed at 72 hours after treatment. The pathological analysis of the lungs of mice was performed immediately. The expression of T-bet and GATA3 protein were detected by RT-PCR. The content of IL-12 in BALF was detected by ELISA and the expression of IL-4 and INF-γ in spleen cells were detected by flow cytometry. Results: The levels of T-bet and IL-12 (P <0.05, P <0.01) were significantly increased and GATA3 was significantly decreased in the lungs of mice in both model group and each administration group. Conclusion: Qingfei Oral Liquid can regulate the balance of TH1 / TH2 and promote the expression of T-bet and IL-12 cytokines in mice infected with rsv, but has obvious inhibitory effect on GATA3, which further proves that Qingfei oral Liquid treatment of RSV viral pneumonia effectiveness.