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从不同地点、不同时间采集的多花筋骨草植株不同部位中提取出昆虫蜕皮激素类似物,通过向幼虫人工饲料中添加昆虫蜕皮激素类似物的方法,研究了筋骨草中植物源蜕皮激素对杨干象2龄幼虫的杀虫作用。实验结果表明:(1)在人工饲料中加入1-3 ml的筋骨草提取物,在24天内可以杀死 58%~100%的幼虫;(2)从开花前的筋骨草中提取出的物质的杀虫效果明显优于从花期和开花后的植物中提取出的物质的杀虫作用, 其1 mL、2 mL和3 mL剂量全株提取物的杀虫效果分别达到65.22%、85.07%和98.11%(幼虫校正死亡率);(3)植株的根部提取的昆虫蜕皮激素类似物的杀虫效果要比茎部或叶部提取的好,1 mL、2 mL、3 mL剂量提取物的杀虫效果分别达到100%、98.20%和98.32%;(4)筋骨草提取物的杀虫速度较慢,处理杨干象2龄幼虫16天后杀虫率才超过50%;(5)幼虫取食加有筋骨草提取物的饲料时间长短直接影响幼虫死亡率,16天的取食,幼虫死亡率达到80%以上。筋骨草采集地点和填加植物源蜕皮激素的方式对杀虫效果的影响较小。图3表3参21。
In this paper, the insect ecdysone analogues were extracted from different parts of Fortucara pachyrhizi collected from different locations and different times. The effects of ecdysone on the growth of Aedes albopictus were studied by adding insect ecdysone analogs to artificial feed of larvae. 2nd instar larvae insecticidal effect. The experimental results show that: (1) 1-3 ml of the extract of Radix extract can kill 58% -100% of the larvae within 24 days; (2) The substance extracted from the Radix extract before the flowering Insecticidal effect was significantly better than those extracted from the flowering and flowering plants insecticidal effect of 1 mL, 2 mL and 3 mL doses of the whole plant extract insecticidal effect reached 65.22%, 85.07% and 98.11% (mortality corrected by larvae); (3) Insect ecdysone analogues extracted from the roots of the plants had better insecticidal effects than stems or leaves, and the killing rates of 1 mL, 2 mL and 3 mL dose extracts (4) the insecticidal speed of Buchongcao extract was slower than that of the second instar larvae, and the insecticidal rate was only over 50% after the second instar larvae were treated for 16 days; (5) Radix extract of the feed time directly affect the mortality of larvae, feeding 16 days, larvae mortality rate of 80% or more. Bugle grass collecting sites and adding plant-source ecdysone have little effect on insecticidal effect. Figure 3 Table 3 Reference 21.