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目的 研究乳腺癌组织中血管生成的定量分析及与肿瘤相关巨噬细胞 (TAMs)的相关性 ,探讨 TAMs对肿瘤间质血管生成的作用机制及对肿瘤生物学行为的影响。方法 应用免疫组化 S- P法检测 5 8例乳腺癌组织中微血管密度 (MVD)、血管内皮细胞生长因子 (VEGF)及 TAMs的分布 ,并用 VIDAS2 1计算机图像分析系统进行定量和相关性分析。结果 乳腺癌组织中微血管分布于肿瘤生长活跃的间质中。VEGF在正常的乳腺组织及癌组织均有表达 ,分布弥散。癌组织及癌周明显高于正常组织 (P<0 .0 1) ,以癌间质最高。TAMs在肿瘤组织内形态呈多样化 ,分布不均 ,在癌间质中最多。 TAMs分布与 VEGF及 MVD呈正相关 (r=0 .5 3,P<0 .0 5 ;r=0 .5 8,P<0 .0 5 )。结论 TAMs广泛浸润肿瘤间质中 ,与 MVD及 VEGF显著相关 ,其在肿瘤微环境中具有促进肿瘤血管生成作用 ,且影响肿瘤的生物学行为。
Objective To investigate the correlation between TAMs and tumor angiogenesis in breast cancer and its relationship with tumor biological behavior. Methods The distribution of microvessel density (MVD), vascular endothelial growth factor (VEGF) and TAMs in 58 cases of breast cancer tissues were detected by immunohistochemical S-P method. Quantitative and correlation analysis was performed by VIDAS2 1 computerized image analysis system. Results The microvessels in breast cancer tissue were distributed in the stroma with active tumor growth. VEGF in normal breast tissue and cancer are expressed, distributed dispersed. Cancer tissue and cancer were significantly higher than the normal tissue (P <0 01), with the strongest of the cancer stroma. TAMs in the tumor tissue morphology was uneven, uneven distribution, the strongest in the cancer stroma. The distribution of TAMs was positively correlated with VEGF and MVD (r = 0.53, P <0.05; r = 0.58, P <0.05). Conclusions TAMs are widely infiltrated into the tumor stroma and have a significant correlation with MVD and VEGF. TAMs can promote tumor angiogenesis in tumor microenvironment, and affect the biological behavior of tumor.