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目的为了检测ＹＹ１在不同宫颈癌细胞及人乳头瘤病毒易感细胞中的表达、功能状态和ＹＹ１位点破坏所诱导的Ｐ９７活性增强效应。方法提取了４种宫颈癌细胞和２种人角源细胞胞核蛋白，检测其内源性ＹＹ１蛋白。同时将带有ＨＰＶ１６标准ＬＣＲ和ＹＹ１位点突变ＬＣＲ序列的荧光素酶质粒短暂转染上述细胞以检测Ｐ９７活性。结果所有被检细胞均含有良好生物学活性的ＹＹ１蛋白，其蛋白含量在各细胞系间无明显差别。ＨＰＶ１６ＬＣＲ上ＹＹ１位点的破坏可在多种细胞，包括人类原代角源细胞中诱导Ｐ９７活性增强。结论表明ＹＹ１蛋白调节系统广泛存于ＨＰＶ易感细胞系内。同时我们还发现转录激活因子ＮＦ１在Ｃ３３ａ细胞中的含量明显高于ＨＴ３细胞，并影响ＹＹ１位点改变所致的Ｐ９７活性增强效应。这提示在不同的细胞系中活性蛋白的表达和含量可能不同
Objective To detect the expression of YY1 in different cervical cancer cells and human papillomavirus susceptible cells, the functional status and YY1 site induced P97 activity-enhancing effect. Methods Four kinds of cervical cancer cells and two kinds of human corneal nucleus protein were extracted and their endogenous YY1 protein was detected. At the same time, luciferase plasmids carrying HPV16 standard LCR and YY1 site mutated LCR sequences were transiently transfected into the above cells to detect P97 activity. Results All the tested cells contained YY1 protein with good biological activity. The protein content of YY1 was not significantly different among all cell lines. The disruption of the YY1 site on HPV16 LCR can induce increased P97 activity in a variety of cells, including human primary keratocytes. Conclusions show that the YY1 protein regulatory system is widely present in HPV susceptible cell lines. At the same time, we also found that the transcriptional activation factor NF1 in C33a cells was significantly higher than that of HT3 cells, and affect YY1 site changes caused by P97 activity enhancement effect. This suggests that the expression and content of active proteins may be different in different cell lines