亲水作用色谱(HILIC)是近年来色谱领域研究的热点之一。本文围绕复杂体系样品中亲水性组分的分离分析,综述了国际上近年来发展的以HILIC为核心的多种液相色谱联用技术及其应用。简要介绍了HILIC的起源、定义、分离特点及其常用固定相;比较了HILIC和反相色谱(RPLC)的选择特性;针对不同层次的分离对象和分离要求,讨论了多种基于HILIC的液相色谱以及液相色谱-质谱联用技术的分离特点和适用范围。 Hydrophilic interaction chromatography (HILIC) is one of the hot topics in the field of chromatography in recent years. In this paper, we focus on the separation and analysis of hydrophilic components in complex system samples, and review the applications and developments of many liquid chromatography combined with HILIC in the world in recent years. The origin, definition, separation characteristics and common stationary phases of HILIC are briefly introduced. The selection characteristics of HILIC and RPLC are compared. According to the different levels of separation objects and separation requirements, many kinds of HILIC-based liquid phase Chromatography and Liquid Chromatography - Mass Spectrometry and the separation of the characteristics and scope of application.