本文报道四氯化碳等6种化学物质对大鼠游离肝细胞染毒后,以酶组织化学、上清液酶活性和台盼蓝排斥试验三类指标对其毒性进行的对比研究。结果表明:台盼蓝排斥试验虽然快速、简便,但不够敏感;不同毒物引起上清液酶活性改变有升高和降低两种类型,故原“逸出酶”的概念应加以修正,并改称为“上清液酶活性”;酶组化不仅较敏感,而且有利于阐明作用机理。 This paper reports six carbon tetrachloride and other chemicals on rat free hepatocytes after exposure to enzyme histochemistry, supernatant enzyme activity and trypan blue exclusion test of three indicators of toxicity compared. The results showed that trypan blue exclusion test was rapid and simple, but not sensitive enough. The changes of enzyme activity in supernatant caused by different poisons had two types of increase and decrease, so the concept of “escaping enzyme” should be amended and renamed As “supernatant enzyme activity”; enzyme grouping is not only more sensitive, but also help clarify the mechanism of action.