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本文采用放射配体受体结合分析研究了HCG对小鼠全脑、卵巢及子宫GABA受体与外源性GABA结合的影响.皮下注射HCG对21天龄性不成熟雌性小鼠连续7天,可促进性腺发育使子宫及卵巢增重,但对GABA受体结合水平有明显的抑制作用.正常组小鼠全脑、卵巢、子宫中GABA受体结合数量比例为1:6:3.以卵巢为最高.与实验组相比,HCG抑制卵巢、子宫及全脑GABA受体结合能力分别为17％,50％及75％,抑制作用以全脑为最强.但HCG注射天数与GABA受体结合的抑制效应之间无明显关系.35天龄性成熟小鼠在皮下注射HCG的同时,使用bicuculline及baclofen观察GABA受体亚型结合变化中发现,脑室注射bicuculline使全脑GABA受体结合能力增加,但子宫与卵巢作用下降.脑室注射baclofen使全脑、卵巢与子宫的GABA受体结合能力都有明显下降.说明在中枢及外周组织中都存在GABA_A与GABA_B受体,但其含量是不均一的。
Radioligand receptor binding assay was used to study the effect of HCG on the binding of GABA receptor to exogenous GABA in the whole brain, ovary and uterus of mice.HCI injected subcutaneously on 21-day-old immature female mice for 7 days, Can promote the gonadal development of the uterus and ovary, but the level of GABA receptor binding significantly inhibited the normal group of mice brain, ovarian and uterine GABA receptor binding number ratio of 1: 6: 3 ovarian Compared with the experimental group, HCG inhibited ovarian, uterine and whole brain GABA receptor binding capacity were 17%, 50% and 75%, the inhibition of the whole brain strongest.But HCG injection days and GABA receptor Binding inhibitory effect between the no significant relationship.35-day-old mature mice injected subcutaneously HCG at the same time, using bicuculline and baclofen GABA receptor subtypes observed changes in binding, intraventricular bicuculline injection of the whole brain GABA receptor binding capacity Increased, but the uterus and ovary decreased.After intravenous injection of baclofen, the binding capacity of GABA receptor in whole brain, ovary and uterus decreased obviously, which indicated that both GABA_A and GABA_B receptors existed in the central and peripheral tissues, but their contents were not evenly.