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株型对水稻产量有着重要影响。作为影响株型的重要因素之一,分蘖角度是水稻高产育种上考虑的重要农艺性状。散生突变体tag1(tiller angle 1)在水稻品种台北309转基因后代中被发现。与野生型相比,主要突变表型为分蘖角度和叶夹角显著增大,并伴随株高、穗长、结实率和枝梗数等性状上的变化。遗传分析表明,tag1的突变性状由一对隐性基因控制。利用F2(tag1/明恢63)群体作为定位群体,突变基因被定位在第11染色体长臂In Del标记AC35795和M7之间,遗传距离分别为0.38 c M和0.95 c M。在该区间内存在一个已被克隆的控制水稻分蘖角度的基因LAZY1。测序比对分析发现,突变体的TAG1存在一个119 bp的缺失,包括第4个外显子50 bp,第4个内含子69 bp,及二者间的剪切位点。因此TAG1是LAZY1的一个新的等位基因,重新命名为LAZY1-2。lazy1和tag1在突变表型上存在一定差异,这可能是LAZY1基因序列的突变位点不同造成的。
Plant type has an important impact on rice yield. As one of the important factors affecting plant type, tillering angle is an important agronomic trait considered in high-yielding rice breeding. Toxoplasma mutant tagl (tiller angle 1) was found in the rice variety Taipei 309 transgenic offspring. Compared with the wild type, the main mutant phenotypes were the tiller angle and the angle between leaves significantly increased, accompanied by changes in plant height, spike length, seed setting rate and branch number. Genetic analysis showed that the mutation of tag1 was controlled by a pair of recessive genes. The F2 (tag1 / Minghui63) population was used as the locating group. The mutant gene was located between the In Del marker AC35795 and M7 on chromosome 11 with genetic distances of 0.38 cM and 0.95 cM, respectively. Within this interval there is a gene that has been cloned to control rice tillering angle, LAZY1. Sequencing analysis showed that there was a 119 bp deletion in TAG1, including 50 bp in the fourth exon and 69 bp in the fourth intron, as well as the cleavage site between them. TAG1 is therefore a new LAZY1 allele, renamed LAZY1-2. lazy1 and tag1 mutations in the phenotype there are some differences, which may be caused by different LAZY1 gene sequence mutation sites.