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利用稀释平板法与Biolog-Eco技术对桑树单作、桑树-大豆间作的桑园土壤微生物数量、种群结构以及微生物碳代谢多样性进行研究,为桑树合理间作其它作物改良桑园土壤提供理论依据。桑树间作大豆的桑园,桑树根际(IMR)土壤中的细菌和放线菌数量、可培养细菌种群结构、微生物碳代谢活性的平均单孔颜色变化率(AWCD)及多样性指数(H)均显著高于单作桑园,而真菌数量低于单作桑园。争论贪噬菌(Variovorax paradoxus)和沙福芽孢杆菌(Bacillus safensis)是间作桑园的桑树根际土壤中特有的优势菌属。土壤微生物对不同碳源的利用及主成分分析表明,糖类、氨基酸、聚合物和混合物是间作桑园桑树根际土壤微生物利用的主要碳源类型,利用强度均显著高于单作桑园的桑树根际土壤微生物。间作桑园土壤的有机质、速效磷和速效钾含量亦显著高于单作桑园。以上研究结果表明,桑树间作大豆改变了桑树根际土壤微生物群落结构组成,增强了根际土壤微生物群落的碳代谢多样性,改善了土壤养分条件。
Using dilution plate method and Biolog-Eco technology, the number of mulberry soil, population structure and microbial carbon metabolism in mulberry monoculture and mulberry-soybean intercropping were studied to provide the theoretical basis for improving the mulberry soil by intercropping mulberry with other crops. The number of bacteria and actinomycetes in mulberry intercropping soybean, mulberry rhizosphere soil (IMR), the average number of single-hole color change rate (AWCD) and diversity index (H) of bacterial population structure and microbial carbon metabolic activity, Were significantly higher than monoculture mulberry, while the number of fungi is lower than monoculture mulberry. Argument that Variovorax paradoxus and Bacillus safensis are dominant dominant species in mulberry rhizosphere soils for intercropping mulberry. Utilization and principal component analysis of different carbon sources by soil microorganisms showed that carbohydrates, amino acids, polymers and mixtures were the main types of carbon sources for soil microbial utilization in mulberry mulching rhizosphere, and their utilization intensity was significantly higher than that of single mulberry Mulberry rhizosphere soil microbe Intercropping mulberry soil organic matter, available phosphorus and available potassium content was significantly higher than single mulberry. The above results show that the intercropping of mulberry with soybean has changed the composition of soil microbial community structure, increased the carbon metabolism diversity of rhizosphere soil microbial community and improved the soil nutrient conditions.