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巨噬细胞是一种重要的免疫活性细胞,在特异和非特异性免疫中都起着十分重要的作用。欲精确地研究巨噬细胞的多种免疫功能,必须采用纯化的巨噬细胞。因此巨噬细胞的分离纯化乃是免疫学中的一项基本技术。巨噬细胞在血清被复的平皿上能发生主动粘附,这种粘附依赖于温度和二价阳离子。先使巨噬细胞粘附于血清被复的平皿上,反复漂洗去除未粘附的细胞,然后在低温下加入乙二胺四乙酸二钠除去二价阳离子,使粘附细胞脱落,从而获得纯化的巨噬细胞。
Macrophages are important immunocompetent cells that play an important role in both specific and nonspecific immunity. To accurately study a variety of immune function of macrophages, you must use purified macrophages. Therefore, the isolation and purification of macrophages is a basic technique in immunology. Macrophages can actively attach to serum-conditioned dishes, depending on temperature and divalent cations. Macrophages are first attached to serum-plated plates, rinsed repeatedly to remove non-adherent cells, and then divalent cations are removed by disodium ethylenediaminetetraacetate at low temperature to detach the adherent cells to obtain purified Of macrophages.