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目的 探讨皮肌炎皮损、肌细胞及浸润淋巴细胞凋亡的表达及皮质类固醇激素的影响。方法 对 2 2个标本用 TU NEL 原位末端标记法检测角质形成细胞、肌细胞及淋巴细胞凋亡。 10例用皮质激素者为 A组 ,12例未用者为 B组。结果 A组角质形成细胞、肌细胞及淋巴细胞有较多的凋亡 ,凋亡指数 (AI)明显大于 B组(P<0 .0 5 )。皮质类固醇激素使用时间在 2周以内者 ,凋亡细胞较少。在观察中未发现凋亡与皮肤、肌肉病理损害程度有关。结论 皮质类固醇激素可能具有诱导角质形成细胞、肌细胞及淋巴细胞凋亡的作用 ,这可能是皮质类固醇激素的治疗机理之一。激素使用时间的长短似与细胞凋亡程度有关。
Objective To investigate the expression of apoptosis in dermatomyositis, myocytes and infiltrating lymphocytes and the effects of corticosteroids. Methods Twenty-two specimens were examined for apoptosis of keratinocytes, myocytes and lymphocytes by TU NEL in situ end labeling. 10 patients with corticosteroid were A group, 12 patients were not used for the B group. Results A group of keratinocytes, myocytes and lymphocytes more apoptosis, apoptosis index (AI) was significantly greater than the B group (P <0.05). Corticosteroid use less than 2 weeks, less apoptotic cells. Apoptosis was not found in the observation and skin, muscle pathological damage related. Conclusion Corticosteroids may induce the apoptosis of keratinocytes, myocytes and lymphocytes, which may be one of the mechanisms of corticosteroid therapy. The duration of hormone use and the degree of apoptosis related.