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茎椰菜(Brassia oleracea var.italica)是国际市场上畅销的高级蔬菜。目前,上海地区生产用种都是从国外进口的F_1种子,不能留种应用。在培育我国自己的品种中,为了利用组织培养技术来加快自交系、自交不亲和系的繁殖和进行春季单株留种,同时对大量繁殖F_1试管苗的方法作些探索,进行了茎椰菜试管苗繁殖的初步研究,探索了生长素和细胞分裂素种类、浓度和配比对花器官的愈伤组织分化的影响及加速试管苗繁殖的方法。
Brassia oleracea var.italica is a top-selling premium vegetable on the international market. At present, the production of seeds in Shanghai are all imported from abroad F_1 seeds, can not be used for seed. In cultivating our own varieties, in order to accelerate the propagation of inbred lines, self-incompatible lines and stay single species in spring in order to make use of tissue culture technology, we explored the method of multiplying F 1 plantlets in large quantities. Preliminary studies on the multiplication of vitro seedlings of broccoli were carried out. The effects of the kinds, concentrations and proportions of auxin, cytokinin on callus differentiation of floral organs and the methods of accelerating the multiplication of plantlets were explored.