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目的探讨多肿瘤抑制基因1(MTS1)和环氧合酶-2(COX-2)基因在浸润性乳腺癌组织中的表达及其临床意义。方法应用流式细胞术(FCM)定量分析66例原发性浸润性乳腺癌患者癌组织、相应癌旁组织中MTS1和COX-2的表达。结果浸润性乳腺癌组织中MTS1和COX-2蛋白表达的相对荧光强度(RFI)中位数分别为0．84和10．54,而相应癌旁组织分别为1．61和4．00,癌组织MTS1和COX-2蛋白表达与相应癌旁组织比较,差异有统计学意义(P<0．05)。MTS1和COX-2在不同年龄、病理类型、肿瘤大小和临床分期间的表达差异均无统计学意义(P>0．05)。有淋巴结转移的患者MTS1和COX-2表达中位数分别为1．12和5．94,无淋巴结转移患者分别为0．79和13．05,差异均有统计学意义(P<0．05)。结论MTS1和COX-2在乳腺癌的发生、发展中起重要作用,并与淋巴结转移状况有关,可作为预测乳腺癌预后的指标。
Objective To investigate the expression of multiple tumor suppressor gene 1 (MTS1) and cyclooxygenase-2 (COX-2) gene in invasive breast cancer and its clinical significance. Methods Flow cytometry (FCM) was used to quantitatively analyze the expression of MTS1 and COX-2 in 66 cases of primary invasive breast cancer and their adjacent tissues. Results The median relative fluorescence intensity (RFI) expression levels of MTS1 and COX-2 protein in invasive breast cancer tissues were 0.84 and 10.54, respectively, while the corresponding paracancerous tissues were 1.61 and 4.00 respectively There were significant differences in the expression of MTS1 and COX-2 between adjacent tissues and adjacent tissues (P <0.05). There was no significant difference in the expression of MTS1 and COX-2 between different age, pathological type, tumor size and clinical stage (P> 0.05). The median MTS1 and COX-2 expression in patients with lymph node metastasis were 1.12 and 5.94, respectively, and those without lymph node metastasis were 0.79 and 13.05, respectively (all P <0.05 ). Conclusions MTS1 and COX-2 play an important role in the occurrence and development of breast cancer and are related to lymph node metastasis, which can be used as an index to predict the prognosis of breast cancer.