一、薄层色谱法的原理在吸附柱色谱法中,被测试样各组分通过在吸附剂上吸附与解吸的反复过程而得到分离。薄层色谱法(TLC)也是根据同样的原理实现分离的,只是吸附剂不在色谱柱中,而是涂在薄层板上。将试样点在薄层板的吸附层上(称为点样,此点称原点),然后使一定的溶剂系统通过毛细作用从不同方向浸润薄层板,带动试样在薄层板上迁移。这个迁移过程实际上是试样中各组分在吸附剂和溶剂系统之间连 First, the principle of thin-layer chromatography In the adsorption column chromatography, the components of the test sample through the adsorbent on the adsorption and desorption repeated the process was isolated. Thin-layer chromatography (TLC) is also based on the same principle to achieve separation, but the adsorbent is not in the column, but coated on the TLC plate. The sample points in the adsorption layer on the thin plate (called the point, this point called the origin), and then make a certain solvent system by capillary action from different directions infiltration of thin plate, led the sample migration in the thin plate . The migration process is actually where the components of the sample are connected between the sorbent and the solvent system