本文用改良的联合培养法观察去传入猫脊髓背角提取液电泳带相对迁移率（Rf）0.11－0.12，0．45－0．48该两蛋白带对鸡胚背根节神经元存活的影响．结果显示：加入手术侧组Rf0.11－0.12，0．45～0．48蛋白带凝胶碎块的培养，48h神经元存活率分别为0．82±0．05、0．81±0．02；而非手术侧组（其神经元存活率）分别为0．55±0．07、0.54±0.08。分别比较两侧各对应组的细胞存活率，均有显著性差异（P＜0．01）。说明手术侧组Rf0．11—0．12及Rf0．45—0．48两蛋白带内均含有促进神经元存活的神经营养活性物质。 In this paper, the modified co-culture method was used to observe the relative mobility (Rf) of the electrophoresis bands extracted from the dorsal horn of cat spinal cord to the cats 0.11-0.12,0.45-0.48. influences. The results showed that the survival rate of neurons at 48h was 0.82 ± 0.05 and 0.81 ± 0 respectively after adding Rf0.11-0.12,0.45-0.48 protein gel fragments. 02; while the non-surgical side group (the survival rate of neurons) were 0.55 ± 0.07,0.54 ± 0.08. Comparing the cell survival rates of the corresponding groups on both sides respectively, there was a significant difference (P <0.01). It shows that the neurotrophic substances that promote the survival of neurons are contained in the two protein bands of Rf0.11-0.12 and Rf0.45-0.48 respectively.