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本文应用超微结构酶细胞化学方法对14例胃癌细胞和1例正常胃粘膜上皮细胞ALPase、ACPase、G6Pase、TPPase和CCOase等五种细胞器标志酶进行了超微结构水平的定位观察,结果表明:1.正常胃粘膜上皮无ALPase反应,ACPase、G6Pase、TP-Pase和CCOase均见于相应细胞器,与其功能代谢相一致。2.部分管状腺癌细胞AL-Pase定位于腔缘及肠型微绒毛,提示它们可能来源于肠化上皮或癌细胞具有向肠上皮分化的倾向;部分管状腺癌细胞ALPase无反应,但保留着胃粘膜上皮的某些特征,表明可能来源于胃固有粘膜上皮。粘液腺癌TPPase和ACPase反应明显,其高尔基体较发达,浣酶体泌噬作用活跃,说明其分泌和消化功能均较强。低分化腺癌各标志酶无反应或反应微弱,与其相应细胞器功能低下或结构异常有关。
In this paper, ultrastructural enzymatic cytochemical methods were used to observe the ultrastructural level of five cell organizing enzymes, including ALPase, ACPase, G6Pase, TPPase, and CCOase in 14 gastric cancer cells and 1 normal gastric epithelial cell. The results showed that: 1. There was no ALPase reaction in normal gastric epithelium, and ACPase, G6Pase, TP-Pase and CCOase were all found in the corresponding organelles, consistent with their functional metabolism. 2. Partial tubular adenocarcinoma cells were localized in the luminal and intestinal microvilli, suggesting that they may be derived from the intestinal epithelium or the cancer cells have a tendency to differentiate into intestinal epithelium; some tubular adenocarcinoma cells have no response to ALPase but remain Some features of gastric mucosal epithelium suggest that it may originate from the inherent mucosal epithelium of the stomach. The TPPase and ACPase responses of mucinous adenocarcinoma are obvious, the Golgi apparatus is more developed, and the zymosomal body’s phagocytosis is active, indicating that the secretion and digestion functions are both strong. The poorly differentiated adenocarcinomas showed no response or weak response to each marker enzyme, which was related to the dysfunction of its corresponding organelles or structural abnormalities.