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我们使用一些酸性试剂及药物,如HCl:谷胱甘肽(GSH,GSSG);维生素C;维生素B_6等,与碱性试剂(Tris)来调节晶体水溶性和尿素溶性蛋白质的pH值,发现在一定比例下,晶体蛋白质溶液产生混浊,用pH计测混浊溶液的pH值约为5.6—6.3,高于或低于这一范围,混浊不会产生.通过酸、碱梯度溶液试验,测定了人类胚胎晶体、成人透明和白内障晶体水溶性和尿素溶性蛋白质溶液的混浊程度,其结果是,成人透明晶体和白内障晶体蛋白溶液比胚胎晶体蛋白溶液产生的混浊明显,在同一晶体中,尿素溶性蛋白比水溶性蛋白产生的混浊多.我们认为白内障的形成与晶体内pH变化有一定联系.
We use a number of acidic reagents and drugs such as HCl: Glutathione (GSH, GSSG); Vitamin C; Vitamin B_6 and so on, and alkaline reagents (Tris) to adjust the crystal water-soluble and urea-soluble protein pH value found in Under certain conditions, the crystal protein solution is turbid, the pH value of the turbid solution measured by pH is about 5.6-6.3, and the turbidity will not be generated above or below this range. By acid and alkali gradient test, Embryonic crystals, the degree of opacity of water-soluble and urea-soluble protein solutions of adult transparent and cataract crystals, as a result, the opacification of adult clear and cataract crystal protein solutions is more pronounced than that of embryonic crystal protein solutions. In the same crystal, the ratio of urea-soluble protein Water-soluble protein produced more turbidity.We believe that the formation of cataract and intragranular pH changes have a certain relationship.