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目的:研究柴胡皂苷A的降温作用及其胞浆内的信号转导机制。方法:以IL-1β致热大鼠为研究对象,采用Elisa、免疫组化的方法检测血清中cAMP含量及下丘脑中PKA的表达情况。结果:(1)侧脑室注射0.15μg IL-1β诱导发热大鼠的血清中cAMP含量明显高于对照组(P<0.01);(2)侧脑室注射0.1μg柴胡皂苷A引起发热大鼠体温降低时,血清中cAMP含量明显低于模型组(P<0.05);(3)模型组胞浆内PKA的表达明显高于对照组,柴胡皂苷A组胞浆内PKA的表达明显低于模型组(P<0.05)。结论:柴胡皂苷A降低IL-1β的致热作用可通过降低下丘脑cAMP的分泌和PKA的活性,抑制胞浆信号转导通路PKA系统实现。
Objective: To study the cooling effect of saikosaponin A and its signal transduction mechanism in cytoplasm. Methods: The IL-1β-induced hyperthermic rats were used as the research objects. Elisa and immunohistochemistry were used to detect the content of cAMP and the expression of PKA in the hypothalamus. Results: (1) The level of cAMP in sera of rats with fever induced by intracerebroventricular injection of 0.15μg IL-1β was significantly higher than that of the control group (P <0.01). (2) The intracerebroventricular injection of 0.1μg saikosaponin A (3) The expression of PKA in the cytoplasm of the model group was significantly higher than that of the control group, and the cytoplasmic PKA expression in the saikosaponin A group was significantly lower than that of the model group (P <0.05) Group (P <0.05). Conclusion: Saikosaponin A can reduce the pyrogenic effect of IL-1β by inhibiting the secretion of cAMP and the activity of PKA in the hypothalamus, and inhibiting the PKA system of cytoplasmic signaling pathway.