目前 ,常规形态学方法、免疫学方法、细胞遗传学方法及ＤＮＡ杂交技术等很难发现少于１ %～５%的微小残留病灶 ,且仪器检测误差较大。山西省肿瘤医院血液科张巧花主任提取实施过自体骨髓移植术的恶性淋巴瘤患者的ＤＮＡ ,采用聚合酶链反应和银染色法进行基因克隆重排的检测 ,通 At present, it is difficult to find the minimal residual lesions of less than 1% to 5% by conventional morphological methods, immunological methods, cytogenetic methods, and DNA hybridization techniques, and the instrumental detection errors are large. Zhang Qiaohua, Director of Department of Hematology, Shanxi Provincial Cancer Hospital, extracted the DNA of patients with malignant lymphoma who had undergone autologous bone marrow transplantation and used polymerase chain reaction and silver staining to detect the gene cloning and rearrangement.