论文部分内容阅读
[目的]考察丹参酮ⅡA(TSⅡA)对白细胞介素-1β(IL-1β)诱导的兔纤维环细胞能量代谢障碍的保护作用。[方法]藻酸盐串珠立体培养兔纤维环细胞,将细胞分为7组,在培养过程中加入不同浓度的药物:A组为空白对照不加入药物,B组加入4μg/mlTSⅡA,C组加入10ng/mlIL-1β,D～G组在给予10ng/mlIL-1β同时分别加入0.5、1、2和4μg/mlTSⅡA。于培养3d后行Na+-K+-ATP酶活性检测、,琥珀酸脱氢酶活性检测、MTT法细胞增殖情况检测以及细胞凋亡的流式细胞仪检测。[结果]G组Na+K+ATP酶活性(3.23±0.28U/mgprot)较C组(1.118±0.15U/mgprot)明显增高(P<0.01),与A组接近(3.57±0.15U/mgprot)(P>0.05)。G组琥珀酸脱氢酶活性(12.48±0.97U/mgprot)较c组(3.03±0.60U/mg-prot)明显增高(P<0.01),与A组接近(14.24±1.56U/mgprot)(P>0.05)。G组MTT试验吸光度(0.77±0.06)较C组(0.31±0.07)明显增高(P<0.01),随着TSⅡA浓度的升高,D～G组吸光度随着TsIIA上升而上升。G组细胞死亡细胞比例和凋亡细胞比例分别为21.08±1.46%和8.99±0.33%,均显著低c组(43.11±2.7,P<0.01和11.71±0.32,P<0.01)。[结论]TSIIA能够减轻IL-1β对纤维环细胞能量代谢的抑制作用,从而改善纤维环细胞的增殖、死亡及凋亡。
[Objective] To investigate the protective effect of tanshinone ⅡA (TSⅡA) on the disturbance of energy metabolism in rabbit fibrocystic ring cells induced by interleukin-1β (IL-1β). [Method] Alginate beads were cultured in three-dimensional culture of rabbit fibrocystic ring cells. The cells were divided into 7 groups. Different concentrations of drugs were added in the culture process: group A was blank control without drugs, group B was added with 4μg / ml TSⅡA, group C 10 ng / ml IL-1β, D ~ G groups were treated with 10 ng / ml IL-1β while adding 0.5, 1, 2 and 4 μg / ml TSⅡA respectively. After 3 days of culture, Na + -K + -ATPase activity assay, succinate dehydrogenase activity assay, MTT cell proliferation assay and apoptosis assay were performed by flow cytometry. [Result] The activity of Na + K + ATPase in group G was significantly higher than that in group C (3.23 ± 0.28U / mgprot) (1.118 ± 0.15U / mgprot) (P <0.01) ) (P> 0.05). The succinate dehydrogenase activity in group G (12.48 ± 0.97U / mgprot) was significantly higher than that in group c (3.03 ± 0.60U / mg-prot) and was similar to that in group A (14.24 ± 1.56U / mgprot) P> 0.05). The absorbance of MTT assay in group G (0.77 ± 0.06) was significantly higher than that in group C (0.31 ± 0.07) (P <0.01). With the increase of TS Ⅱ A concentration, the absorbance of group D-G increased with the increase of TsIIA. The percentage of apoptotic cells and apoptotic cells in group G were 21.08 ± 1.46% and 8.99 ± 0.33%, respectively, which were significantly lower in group C than in group C (43.11 ± 2.7, P <0.01 and 11.71 ± 0.32, P <0.01). [Conclusion] TSIIA can reduce the inhibitory effect of IL-1β on the energy metabolism of the fibrocytes, so as to improve the proliferation, death and apoptosis of the fibrocystic ring cells.