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Objective:To investigate the impact of bone marrow mesenchymal stem cells on Smad expression of hepatic fibrosis rats.Methods:A total of 48 adult female SD rats were randomly divided into three groups,normal control group(n=10),observation group(n=19)with liver fibrosis model rats injected with BMSCs cells:model group(n=19),with liver fibrosis model rats injected with physiological saline.Serum index,TGF-β1 and Smad expression were detected.Results:TypeⅢprocollagen,Ⅳcollagen,hyaluronic acid,laminin levels of observation group were significantly lower than those of model group(P<0.05).The content and expression of TGF-β1in serum and liver tissue of observation group were significantly lower than those of model group(P<0.05).Compared with normal control group,the Smad3,Smad4 mRNA and protein expression of model group were significantly increased,the Smad7 mRNA and protein expression were significantly reduced(P<0.05).Compared with model group.Smad3,Smad4 mRNA and protein expression of observation group were significantly reduced,and Smad7 mRNA expression were significantly increased(P<0.05).Conclusions:BMSCs can regulate Smad expression to some extent,and reduce the degree of liver fibrosis.
Objective: To investigate the impact of bone marrow mesenchymal stem cells on Smad expression of hepatic fibrosis rats. Methods: A total of 48 adult female SD rats were differentiated into three groups, normal control group (n = 10), observation group (n = 19) with liver fibrosis model rats injected with BMSCs cells: model group (n = 19), with liver fibrosis model rats injected with physiological saline. Serum index, TGF-β1 and Smad expression were detected. Results: Type Ⅲ proteocollagen, Ⅳ coli, hyaluronic acid, laminin levels of observation group were significantly lower than those of model group (P <0.05). The content and expression of TGF-β1in serum and liver tissue of observation group were significantly lower than those of model group (P <0.05). Compared with normal control group, the Smad3, Smad4 mRNA and protein expression of model group were significantly increased, the Smad7 mRNA and protein expression were significantly reduced (P <0.05) .Compared with model group.Smad3, Smad4 mRNA and protein expression of observation groups were significantly reduced, and Smad7 mRNA expression were significantly increased (P <0.05) .Conclusions: BMSCs can regulate Smad expression to some extent, and reduce the degree of liver fibrosis.