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目的:将尿素酶试剂直接于胃镜下喷洒观察胃黏膜的颜色变化,以期明确Hp分布范围并减少了取材的盲目性,拟在Hp分布的范围内取活组织进一步做病理切片Giemsa染色以检测Hp.方法:选择慢性浅表性胃炎患者47例.实验组,将尿素酶试剂喷洒于胃窦部,变红色的区域为Hp分布区,在此红色部位取活检组织1块.胃黏膜未变红者提示Hp尿素酶试验阴性,于胃窦取活组织1块.对照组,不喷洒尿素酶试剂,于胃窦取活组织1块.上述活组织均用Giemsa染色检测Hp,分别计算两组的Hp阳性率.结果:胃内尿素酶试剂喷洒后阳性者的组织切片Hp检出率为75%,明显高于对照组的检出率25%,P<0.05.胃内尿素酶试剂喷洒后阴性者的组织切片Hp检出率为20%,与对照组的检出率25%相比无显著差异,P>0.05.提示胃内喷洒尿素酶试剂可提高组织切片Hp的检出率.结论:此方法可以明确Hp分布范围并提高了活组织病理检测Hp的阳性率,减少了活组织取材检测Hp的盲目性.
OBJECTIVE: To directly observe the color change of gastric mucosa by spraying urease reagent directly under the endoscope, in order to clarify the distribution of Hp and reduce the blindness of drawing. It is proposed to take Giemsa staining to detect Hp within the range of Hp distribution .Methods: Forty-seven patients with chronic superficial gastritis were selected.In the experimental group, urease reagent was sprayed on the gastric antrum, the reddish area was Hp distribution area, and the red area was taken as the biopsy tissue 1. The gastric mucosa did not turn red Those who Hp urease test negative, taken in the antrum of the gastric tissue 1. The control group, do not spray urease reagent, taken in the antrum of the gastric tissue 1. The living tissue were detected by Giemsa Hp, were calculated in both groups Hp positive rate.Results: The positive rate of Hp detection in gastric tissue was 75%, which was significantly higher than that of control group (P <0.05) .The negative rate of gastric enzyme in urease The detection rate of Hp in tissue sections was 20%, which was not significantly different from the detection rate of 25% in control group (P> 0.05), suggesting that the intragastric administration of urease reagent could improve the detection rate of Hp in tissue sections.Conclusion: This method can be clearly Hp distribution and improve the detection of Hp biopsy pathology The positive rate, reduce the blindness of detection of Hp in biological samples.