论文部分内容阅读
目的探讨人脐带间充质干细胞(umbilical cord-derived mesenchymal stem cells,UCMSCs)多点局部注射后,修复烫伤皮肤创面的潜在机制。方法机械消化法分离人UCMSCs,培养至第3代后,进行形态观察、免疫表型和多向分化潜能鉴定。将第3代UCMSCs与碱性成纤维细胞因子(basic fibroblast growth factor,b FGF)共培养14 d,免疫荧光法检测成纤维细胞标志物波形蛋白的表达;对比小鼠Ⅲ度烫伤模型多点局部注射UCMSCs后创面愈合率及创面愈合时间;Dil标记UCMSCs,局部注射后检测UCMSCs在创面局部的分布情况。结果分离培养的UCMSCs的形态、表面抗原表达及体外诱导分化能力均符合间充质干细胞特性;经b FGF诱导后可显著提高UCMSCs中成纤维细胞标志物波形蛋白的表达量;局部注射UCMSCs可显著提高烫伤小鼠的创面愈合率,UCMSCs可定植于烫伤创面下。结论 UCMSCs具有较强的成纤维样细胞分化能力,经局部注射后,可通过定植于烫伤创面而显著提高创面愈合率。
OBJECTIVE: To investigate the potential mechanism of multi-site local injection of umbilical cord-derived mesenchymal stem cells (UCMSCs) to repair wounds burned on the skin. Methods Human UCMSCs were isolated by mechanical digestion. After passage 3, they were observed for morphology, immunophenotype and multidirectional differentiation potential. The 3rd generation UCMSCs were co-cultured with basic fibroblast growth factor (bFGF) for 14 days, and the expression of fibroblast marker vimentin was detected by immunofluorescence. The wound healing rate and wound healing time after UCMSCs injection were recorded. Dil labeled UCMSCs were used to detect the local distribution of UCMSCs in wounds after local injection. Results The morphology, surface antigen expression and differentiation-inducing ability of UCMSCs in vitro were in accordance with the characteristics of mesenchymal stem cells. The expression of vimentin in UCMSCs was significantly increased after induced by b FGF. UCMSCs could be markedly injected To improve wound healing rate in scalded mice, UCMSCs can be implanted under scald wounds. Conclusions UCMSCs have strong fibroblast-like cell differentiation ability. After local injection, UCMSCs can significantly improve wound healing rate through scald wounds.