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目的 对产超广谱 β-内酰胺酶 (ESBL s)变形杆菌的耐药机制和分子流行病学进行初步探讨。方法 收集、鉴定菌株 ,进行 ESBL s检测、PCR扩增 bla TEM和 bla SHV基因、等电聚焦测定 β-内酰胺酶等电点和染色体DNA的 PFGE分析。结果 分离的 45株变形杆菌中有 7株产 ESBL s,bla TEM和 bla SHV基因扩增和等电聚焦结果显示 7株产 ESBL s变形杆菌全部为 TEM型 β-内酰胺酶 ;PFGE结果时间上和空间上未见有相关菌株的存在或明显的集中趋势。结论 合理使用抗生素对预防耐药菌的产生和控制医院感染十分重要 ;对患有产 ESBL s变形杆菌感染的患者应加强消毒隔离措施 ,防止医院感染的暴发和流行。
OBJECTIVE: To investigate the drug resistance mechanism and molecular epidemiology of ESBLs-producing Proteobacteria. Methods Strains of ESBLs were collected and identified by ESBLs. The genes of bla TEM and bla SHV were amplified by PCR. PFGE analysis of isoelectric point of β-lactamase and chromosomal DNA was carried out by isoelectric focusing. Results Among the 45 strains of Proteus isolated, 7 strains of ESBL-s, bla TEM and bla SHV genes were amplified and isoelectric focusing results showed that all 7 strains of ESBL Proteus were TEM-type β-lactamases; No spatially related strains were present or clearly concentrated. Conclusion The rational use of antibiotics is very important to prevent the emergence of drug-resistant bacteria and to control nosocomial infections. Patients with ESBLs-producing Proteus infection should be strengthened disinfection and isolation measures to prevent the outbreak and epidemic of nosocomial infections.