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本文作者用不同照射量的γ射线照射曼氏血吸虫童虫,经肌肉注射感染小鼠,以观察血吸虫童虫在鼠体内的移行和存活的情况。用 T.O.远系繁殖的第6周雄鼠。虫体的回收是用门脉系统灌注法和肺组织内虫体的回收相结合,灌注液用含25单位/毫升肝素的 Hanks 平衡盐溶液。鼠肺内血吸虫童虫的回收,采用把切细的肺组织经孵育3小时和24小时后收集的方法。用组织压片法镜检肝组织内的虫卵,并用 Doenhoff 等(1978) 所描述的方法推算虫卵数。根据卵的孵化力确定卵的活力。成虫用乙酰-明矾胭脂红染色以观察生殖器官。
In this study, the authors inoculated Schistosoma mansoni Schistosoma japonicum with γ-rays of different doses to infect mice via intramuscular injection to observe the migration and survival of Schistosoma japonicum in mice. Week 6 males were bred with T.O. The recovery of the parasites was performed by a portal system perfusion method combined with recovery of parasites in the lung tissue. The perfusate was treated with a Hanks balanced salt solution containing 25 units / ml heparin. Schistosoma japonicum Schistosomiasis for the recovery of worms, using shredded lung tissue incubated for 3 hours and 24 hours after the collection method. Liver tissues were microscopically examined for ovulation by tissue compression and the number of eggs was calculated using the method described by Doenhoff et al. (1978). According to the hatching power of eggs to determine the vitality of eggs. Adults were stained with acetyl-alum carmine to observe the reproductive organs.