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目的测定粪肠球菌的耐药情况,了解污染肉食品的肠球菌的耐药现状。方法从各集贸市场的零售鲜肉和熟肉制品中分离提纯粪肠球菌,采用K-B纸片扩散法进行药物敏感性分析。结果 47株粪肠球菌对头孢氨噻肟、四环素、卡那霉素、复方新诺明、新霉素耐药性较高,耐药率分别达到86.1%、63.4%、60.0%、56.8%、55.0%;对氯霉素、替考拉宁、呋喃妥因、氨苄青霉素敏感性较高,敏感率分别达到了77.5%、76.1%、74.4%、73.8%,但也发现了对替考拉宁和高水平链霉素、庆大霉素耐药性菌株,这在非医院内感染的肠球菌菌株中尚未见报道。通过对两种不同来源的粪肠球菌的药物敏感性分析,两种不同来源的粪肠球菌对22种抗生素的耐药性有所不同,均出现了糖肽类抗生素抗性菌株,且鲜肉样品中还出现了四株高水平链霉素抗性菌株和两株高水平庆大霉素抗性菌株。另外,对临床常用的青霉素和氟哌酸等抗性,鲜肉也比熟肉分离菌株高。结论粪肠球菌表现为多重耐药。
Objective To determine the drug resistance of Enterococcus faecalis and to understand the drug resistance of enterococci contaminated with meat products. Methods The Enterococcus faecalis was isolated and purified from the retail fresh meat and cooked meat products of each bazaar, and drug susceptibility analysis was performed by K-B disk diffusion method. Results 47 strains of Enterococcus faecalis were highly resistant to cefotaxime, tetracycline, kanamycin, cotrimoxazole and neomycin, with the rates of resistance reaching 86.1%, 63.4%, 60.0% and 56.8% respectively, 55.0%. The sensitivity to chloramphenicol, teicoplanin, nitrofurantoin and ampicillin were higher, and the sensitivity rates were 77.5%, 76.1%, 74.4% and 73.8% respectively, but the sensitivity to teicoplanin and high The level of streptomycin, gentamycin-resistant strains, which have not been reported in non-nosocomial enterococcal strains. Through the drug sensitivity analysis of two different sources of Enterococcus faecalis, two different sources of Enterococcus faecalis have different resistance to 22 kinds of antibiotics, all appeared glycopeptide antibiotic resistant strains, and fresh meat Four high-level streptomycin-resistant strains and two high-level gentamycin-resistant strains also appeared in the samples. In addition, common clinical penicillin and norfloxacin resistance, fresh meat than cooked meat isolates. Conclusion Enterococcus faecalis showed multi-drug resistance.