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目的:探讨急性冠脉综合征患者胸腺新近输出的CD4+CD25+调节性T细胞(Treg)的水平及意义。方法:实验共分3组:急性冠脉综合征(ACS)组28例,稳定型心绞痛(SA)组25例和健康对照(HCs)组24例。采用CD31作为胸腺新近输出的典型标志,用磁性细胞分离器(MACS)分离各组CD4+CD25+调节性T细胞,流式细胞分析法检测各组患者外周血CD31+Treg占Treg细胞的比例,应用实时定量PCR检测CD4+CD25+调节性T细胞中T细胞受体重排删除环的数量。结果:ACS组外周血CD31+Treg/Treg比例明显低于SA组(P=0.003)和HCs组(P<0.001)。ACS组Treg细胞中TREC的数量亦明显低于SA组(P=0.001)和HCs组(P<0.001)。而SA组和HCs组间差异均无统计学意义(P=0.106和P=0.566)。患者CD31+Treg的比例与Treg细胞中TREC的数量呈正相关(r=0.493,P=0.014)。结论:急性冠状动脉综合征患者胸腺新近输出CD4+CD25+调节性T细胞减少可能是动脉粥样斑块不稳定的原因。
Objective: To investigate the level and significance of CD4 + CD25 + regulatory T cells (Tregs) newly released from thymus in patients with acute coronary syndrome. Methods: The experiment was divided into 3 groups: 28 cases of acute coronary syndrome (ACS), 25 cases of stable angina (SA) and 24 cases of healthy control (HCs). Using CD31 as a typical marker of thymus output, each group of CD4 + CD25 + regulatory T cells was separated by magnetic cell separator (MACS). The proportion of CD31 + Treg in Treg cells in peripheral blood was measured by flow cytometry Real-time quantitative PCR was used to detect the number of T-cell receptor rearrangement deletion loops in CD4 + CD25 + regulatory T cells. Results: The percentage of CD31 + Treg / Treg in peripheral blood of ACS group was significantly lower than that of SA group (P = 0.003) and HCs group (P <0.001). The number of TREC in Treg cells in ACS group was also significantly lower than that in SA group (P = 0.001) and HCs group (P <0.001). There was no significant difference between SA group and HCs group (P = 0.106 and P = 0.566). The proportion of patients with CD31 + Treg was positively correlated with the number of TRECs in Treg cells (r = 0.493, P = 0.014). Conclusion: The decrease of CD4 + CD25 + regulatory T cells in thymus of patients with acute coronary syndrome may be the cause of atherosclerotic plaque instability.