【摘 要】
：
An E.coli SOS-EGFP biosensor which expresses enhanced green fluorescent protein as a reporter protein under the control of recA gene promoter in SOS response wa
【机 构】
：
Research Center for Eco-Environmental Sciences
【基金项目】
：
国家自然科学基金;the National Basic Research Program(973)of China
论文部分内容阅读
An E.coli SOS-EGFP biosensor which expresses enhanced green fluorescent protein as a reporter protein under the control of recA gene promoter in SOS response was constructed for detection of DNA damage and evaluation of DNA damaging chemicals.The chemicals that may cause substantial DNA damage will trigger SOS response in the constructed bacterial biosensor,and then the reporter egfp gene under the control of recA promoter is stimulated to express as a fluorescent protein,allowing fast and sensitive fluorescence detection.Interestingly,this biosensor can be simultaneously applied for evaluation of genotoxicity and cytotoxicity.The SOS-EGFP bacterial biosensor provides a sensitive,specific and simple method for detecting known and potential DNA damaging chemicals.
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