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目的研究HLA DRB1等位基因的分型配合 ,因为相配的肾移植 ,其急性排斥反应的发生率明显降低 ,移植肾的存活时间明显延长。方法我们合成了一对DR4特异性引物 ,对DR4阳性的肾移植供受者的DNA样品进行PCR扩增后 ,将PCR产物分别用限制性核酸内切酶SacII,AvaII ,HinfI,HaeII ,HphI和MnlI进行酶切。结果根据酶切结果及RFLP图型 ,可将DR4进一步分为DR4 DRB1 0401～0411。结论本方法快速、简便、精确 ,可为肾移植中DR4阳性供受者HLA DRB1等位基因的配型提供可靠的方法。
Objective To study the HLA DRB1 allele typing and matching, because the matching kidney transplantation, the incidence of acute rejection was significantly reduced, the survival time of renal transplant significantly prolonged. Methods We synthesized a pair of DR4-specific primers for PCR amplification of DNA samples of DR4-positive donor kidney recipients. PCR products were digested with restriction endonucleases SacII, AvaII, HinfI, HaeII, HphI and MnlI digestion. Results According to the results of digestion and RFLP patterns, DR4 could be further divided into DR4 DRB1 0401 ~ 0411. Conclusion The method is rapid, simple and accurate and can provide a reliable method for HLA DRB1 allele typing in DR4-positive donors in renal transplantation.