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目的 :观察甘草对大鼠小肠移行性综合肌电 (MMC)的影响 ,并探讨其作用机制。方法 :2 0只SD大鼠平分为氨甲酰甲胆碱组和甘草组 ,用药前后检测小肠的电活动 ,以MMC活动周期、Ⅲ相持续时间为指标分析小肠电活动状态。另2 0只SD大鼠平分为正常组和甘草组 ,用免疫组织化学法检测小肠嗜铬细胞及小肠神经系统 (ENS)中 5 HT的含量 ,用显微分光光度计对免疫染色强度进行定量测定。结果 :(1)甘草能抑制M 受体激动剂氨甲酰甲胆碱引起的十二指肠和空肠收缩反应 ,并能预防其收缩反应的发生 ;(2 )甘草组与正常组比较 ,小肠嗜铬细胞和ENS中 5 HT含量明显减少。结论 :(1)甘草对大鼠十二指肠和空肠的抑制作用机制之一可能是由M 受体介导的突触后抑制作用 ;(2 )甘草通过抑制小肠嗜铬细胞分泌 5 HT及降低ENS中 5 HT的含量而对大鼠小肠MMC活动起抑制作用
Objective: To observe the effects of licorice on transitional complex electromyography (MMC) in rat small intestine and to explore its mechanism of action. Methods : Twenty SD rats were equally divided into bethanechol group and licorice group. Electrical activities of the small intestine were measured before and after treatment. The activity status of the small intestine was analyzed using the MMC activity cycle and duration of phase III as indicators. Another 20 SD rats were equally divided into the normal group and the licorice group. The contents of 5HT in the small intestine chromaffin cells and the small intestine nervous system (ENS) were detected by immunohistochemistry, and the immunostaining intensity was quantified using a microspectrophotometer. Determination. Results: (1) Glycyrrhiza can inhibit duodenal and jejunal contractile responses induced by the M receptor agonist bethanechol and prevent the occurrence of contractile responses; (2) Glycyrrhiza group compared with the normal group, the small intestine The content of 5 HT in chromaffin cells and ENS was significantly reduced. CONCLUSION: (1) One of the mechanisms of inhibition of duodenum and jejunum in rats by licorice may be post-synaptic inhibition mediated by M receptor; (2) licorice inhibits secretion of 5 HT by the chromaffin cells in the small intestine. Reduce the content of 5 HT in ENS and inhibit the activity of MMC in the small intestine of rats