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采用表面分子印迹技术,以谷胱甘肽(GSH)为模板分子,N-乙烯基吡咯烷酮(NVP)和丙烯酰胺(AM)为功能单体,N,N′-亚甲基双丙烯酰胺(MBA)为交联剂,γ-(甲基丙烯酰氧)丙基三甲氧基硅烷(KH570)改性的Fe3O4纳米颗粒为磁性载体,制备了对GSH有特异识别性的磁性分子印迹聚合物(GSH-MMIPs).利用X射线衍射仪(XRD)、透射电子显微镜(TEM)、红外光谱(FT-IR)和振动样品磁强计(VSM)对聚合物进行了表征,结果表明磁性载体表面成功地包覆了分子印迹聚合物薄层.静态吸附平衡实验和Scatchard分析结果表明,GSH-MMIPs中存在两类不同的结合位点,平衡解离常数分别为8.786×10-4 mol/L和5.424×10-3 mol/L,最大吸附量分别为49.195 mg/g和155.003 mg/g.与化学组成相同的磁性非印迹聚合物(GSH-MNIPs)相比,GSH-MMIPs对谷胱甘肽有较高的选择吸附性能.
Using surface molecular imprinting technique, glutathione (GSH) as template molecule, N-vinyl pyrrolidone (NVP) and acrylamide (AM) as functional monomers, N, N’-methylenebisacrylamide ) Was used as crosslinking agent, and the Fe3O4 nanoparticles modified by γ- (methacryloyloxy) propyltrimethoxysilane (KH570) were used as magnetic carriers to prepare magnetic molecularly imprinted polymer (GSH -MMIPs) .The polymer was characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), infrared spectroscopy (FT-IR) and vibrating sample magnetometer (VSM) .The results showed that the magnetic carrier surface successfully Coated molecularly imprinted polymers.The results of static adsorption equilibrium and Scatchard analysis showed that there are two kinds of binding sites in GSH-MMIPs with equilibrium dissociation constants of 8.786 × 10-4 mol / L and 5.424 × 10-3 mol / L, and the maximum adsorption capacity was 49.195 mg / g and 155.003 mg / g, respectively.Compared with GSH-MNIPs with the same chemical composition, GSH-MMIPs had higher glutathione High selection of adsorption properties.